Mouse Monoclonal Anti-Cyclin H Antibody (1H9-D3-E11)

Catalog number
A1320-100
Name
Mouse Monoclonal Anti-Cyclin H Antibody (1H9-D3-E11)
Size
100 µg
Price
338.00 EUR
Supplier

Details

Description
Mouse Monoclonal antibody to detect Cyclin H
Raised in
Mouse
Clonality
Monoclonal Antibody (Clone#1H9-D3-E11)
Reacts with
Human
Immunogen
Recombinant human Cyclin H protein fragments expressed in E.coli
Target
Cyclin-H (Cyclin-H, MO15-associated protein, p34, p37)
Research area
Cell Cycle
Gene number
902
Protein number
P51946
Physical state
Colorless liquid
Package form
Affinity purified antibody is supplied in liquid form in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol
Purity
Affinity purified
Antibody's positive controles
Western blot: Jurkat, K562 and A431 cell lysates; Immunoprecipitation: HeLa cell lysates
Approved applications with antibody dilution
Western blot; 1:1000, Immunoprecipitation
Scientific context
Regulates CDK7, the catalytic subunit of the CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle.
Handling and storage
Before use gently mix the antibody solution. Store this antibody at -20 °C for 12 months. Refer to the label on vial
Delivery
Shipped to your lab under gel pack
Precautions
Manifactured in USA. For Research Use Only! Not to be used in humans
Released
This antibody is available to order since May 2017
Additional description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin name
Mus musculus
French translation
anticorps