Mouse Monoclonal Anti-Hexokinase 1 Antibody (6B8-G11-E7)

Catalog number
A1330-100
Name
Mouse Monoclonal Anti-Hexokinase 1 Antibody (6B8-G11-E7)
Size
100 µg
Price
338.00 EUR
Supplier

Details

Description
Mouse Monoclonal antibody to detect Hexokinase 1
Raised in
Mouse
Clonality
Monoclonal Antibody (Clone#6B8-G11-E7)
Reacts with
Human
Immunogen
Recombinant human Hexokinase 1 protein fragments expressed in E.coli
Target
Hexokinase-1 (Hexokinase-1, Brain form hexokinase, Hexokinase type I, HK I)
Research area
Signal Transduction
Gene number
3098
Protein number
P19367
Physical state
Colorless liquid
Package form
Affinity purified antibody is supplied in liquid form in PBS (pH 7.4) containing with 0.02% sodium azide and 50% glycerol
Purity
Affinity purified
Antibody's positive controles
Western blot: MCF7, Jurkat, CHO-K1, C2C12, C6, 3T3 and Hela cell lysates
Approved applications with antibody dilution
Western blot; 1:1000
Scientific context
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in five transcript variants which encode different isoforms, some of which are tissue-specific. Each isoform has a distinct N-terminus; the remainder of the protein is identical among all the isoforms. A sixth transcript variant has been described, but due to the presence of several stop codons, it is not thought to encode a protein.
Handling and storage
Before use gently mix the antibody solution. Store this antibody at -20 °C for 12 months. Refer to the label on vial
Delivery
Shipped to your lab under gel pack
Precautions
Manifactured in USA. For Research Use Only! Not to be used in humans
Released
This antibody is available to order since May 2017
Additional description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin name
Mus musculus
French translation
anticorps