Mouse Monoclonal Anti-UCHL1 Antibody (7B8-G5-B8)
Catalog number
A1332-100
Name
Mouse Monoclonal Anti-UCHL1 Antibody (7B8-G5-B8)
Size
100 µg
Price
338.00 EUR
Supplier
Details
Description
Mouse Monoclonal antibody to detect UCHL1/PGP9.5
Raised in
Mouse
Clonality
Monoclonal Antibody (Clone#7B8-G5-B8)
Reacts with
Human, Monkey
Immunogen
Recombinant human UCHL1/PGP9.5 protein fragments expressed in E.coli
Target
Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCH-L1, Neuron cytoplasmic protein 9.5, PGP 9.5, Ubiquitin thioesterase L1, Ubiquitin carboxyl-terminal hydrolase isozyme L1)
Research area
CD Antigen
Gene number
7345
Protein number
P09936
Physical state
Colorless liquid
Package form
Affinity purified antibody is supplied in liquid form in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol
Purity
Affinity purified
Antibody's positive controles
Western blot: U251, IMR32, Raji and COS7 cell lysates; Immunofluorescence: COS7 cells
Approved applications with antibody dilution
Western blot; 1:1000, Immunofluorescence; 1:300
Scientific context
Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
Handling and storage
Before use gently mix the antibody solution. Store this antibody at -20 °C for 12 months. Refer to the label on vial
Delivery
Shipped to your lab under gel pack
Precautions
Manifactured in USA. For Research Use Only! Not to be used in humans
Released
This antibody is available to order since May 2017
Additional description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin name
Mus musculus
French translation
anticorps
