Human Beta-lactaminase Recombinant protein
Catalog number
P1196-20
Name
Human Beta-lactaminase Recombinant protein
Size
20 μg
Price
186.00 EUR
Supplier
Details
Scientific context
AmpC, also known as Beta-lactamase, is the most widespread resistance mechanism to beta-lactam antibiotics, such as the penicillins and the cephalosporins. These antibiotics have a common element in their molecular structure: a four-atom ring known as a beta-lactam. The lactamase enzyme breaks that ring open, deactivating the molecule's antibacterial properties. Recombinant E.coli beta-lactamase protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
Specific targetted sequence
MGSSHHHHHH SSGLVPRGSH MAPQQINDIV HRTITPLIEQ QKIPGMAVAV IYQGKPYYFT WGYADIAKKQ PVTQQTLFEL GSVSKTFTGV LGGDAIARGE IKLSDPTTKY WPELTAKQWN GITLLHLATY TAGGLPLQVP DEVKSSSDLL RFYQNWQPAW APGTQRLYAN SSIGLFGALA VKPSGLSFEQ AMQTRVFQPL KLNHTWINVP PAEEKNYAWG YREGKAVHVS PGALDAEAYG VKSTIEDMAR WVQSNLKPLD INEKTLQQGI QLAQSRYWQT GDMYQGLGWE MLDWPVNPDS IINGSDNKIA LAARPVKAIT PPTPAVRASW VHKTGATGGF GSYVAFIPEK ELGIVMLANK NYPNPARVDA AWQILNALQ
Molecular weight (in Da)
41.8 kDa
Protein target
ampC
Other name
ampC, ampA, Cephalosporinase
Gene origin
Human
Gene number
948669 (ampC)
Protein number
P00811
Source
E.coli
Application
SDS-PAGE (Purity > 95% )
Endotoxins
N/A
Specific activity
The specific activity (specifications/test method) was measured by its ability to hydrolyze 1.0 µmole of Nitrocefin per minute at pH 7.0 at 37C.
Bioactivity
The specific bioactivity is >700 units/mg
Example results
Expected results >700 units/mg
Unit Definition
One unit will hydrolyze 1.0 µmole of Nitrocefin per minute at pH 7.0 at 37C.
Handling and storage
Centrifuge the vial prior to opening. Store for 12 months at -20°C
Delivery
Shipped under gel pack
Package form
This product is supplied as liquid form in 20 mM Tris-HCl buffer (pH8.0) containing 10% glycerol
Reconstitute
See included datasheet or contact our technical support
Precautions
Manufactured in USA. For Research Use Only! Not to be used in humans
Released
Manifactured since May 2017. Available to order. Please contact us for larger or bulk quantity
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants
