Human Cyclophilin B Recombinant protein
Catalog number
P1197-20
Name
Human Cyclophilin B Recombinant protein
Size
20 μg
Price
186.00 EUR
Supplier
Details
Scientific context
PHPT1 is a 125 amino acid enzyme belonging to the Janus protein family. Existing as a monomer in the cytoplasm, PHPT1 is an EDTA-insensitive phosphohistidine phosphatase. Overexpression of PHPT1 leads to specific phosphohistidine phosphatase activity towards phosphopeptide I, with no activity detected towards phosphotyrosine, phosphothreonine and phosphoserine peptides. Recombinant human PHPT1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Specific targetted sequence
MGSSHHHHHH SSGLVPRGSH MGSMNDKKKG PKVTVKVYFD LQIGDESVGR VVFGLFGKTV PKTVDNFVAL ATGEKGFGYK NSKFHRVIKD FMIQGGDFTR GDGTGGKSIY GERFPDENFK LKHYGPGWVS MANAGKDTNG SQFFITTVKT SWLDGKHVVF GKVLEGMDVV RKVESTKTDS RDKPLKDVII VDSGKIEVEK PFAIAKE
Molecular weight (in Da)
22.7 kDa
Protein target
Ppib
Other name
AA408962, AA553318, AI844835, Cphn-2, Cphn2, CyP-20b
Gene origin
Mouse
Gene number
19035 (Ppib)
Protein number
P24369
Source
E.coli
Application
SDS-PAGE (Purity > 95% )
Endotoxins
N/A
Specific activity
The specific activity (specifications/test method) was measured by its ability to cleaves 1 nmole of suc-AAFP-PNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.
Bioactivity
The specific bioactivity is >1,000 nmol/min/mg
Example results
Expected results >1,000 nmol/min/mg
Unit Definition
One unit is defined as the amount of enzyme that cleaves 1 nmole of suc-AAFP-PNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.
Handling and storage
Centrifuge the vial prior to opening. Store for 12 months at -20°C
Delivery
Shipped under gel pack
Package form
This product is supplied as liquid form in Phosphate Buffered Saline (pH7.4) containing 10% glycerol
Reconstitute
See included datasheet or contact our technical support
Precautions
Manufactured in USA. For Research Use Only! Not to be used in humans
Released
Manifactured since May 2017. Available to order. Please contact us for larger or bulk quantity
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants
