PCR-Salmonella-Listeria Detection Kit

-20°C

PCR based Salmonella spp & Listeria monocytogenes Detection Kit

RT

12 months

Listeria monocytogenes, Salmonella

Real-time PCR

Food samples, Bacteria, Salmonella spp & Listeria monocytogenes, Beef, milk products, fresh vegetables, and contaminated water

Listeria monocytogenes and Listeria species are gram-positive, rod-shaped, facultatively anaerobic, and non spore-forming. The major human pathogen in the Listeria genus is L. monocytogenes. It is usually the causative agent of the relatively rare bacterial disease listeriotic, listeriosis a serious infection caused by eating food contaminated with the bacteria. The disease affects pregnant women, newborns, adults with weakened immune systems, and the elderly. Antibodies and ELISA test kits for detection of listeria antigens.Salmonella typhimurium, enteriditis and Salmonella paratyphi antibodies or media detect this rod-shaped (bacillus) gram-negative bacteria of the Enterobacteriaceae family. The two species of Salmonella are Salmonella enterica and Salmonella bongori. Salmonella enterica is the type species and is further divided into six subspecies that include over 2500 serovars. S. enterica subspecies are found worldwide in all warm-blooded animals, and in the environment. S. bongori is restricted to cold-blooded animals particularly reptiles. Strains of Salmonella cause illnesses such as typhoid fever, paratyphoid fever, and food poisoning (salmonellosis).

The detections of the targets with this kit is a type of test that can be performed on any target containing biological samples after clean up of interfering agents. The assay must be performed following the protocol.

PCR, polymerase chain reaction

TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.

Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.