TNF-alpha Extracellular Domain, Human Recombinant

TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Cachectin, DIF, TNFA, TNFSF2

TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Cachectin, DIF, TNFA, TNFSF2

A potent cytokine exerting cytotoxic effects on a wide range of cells

7124

TNFA

Human

P01375

False

CHO cells

ED50 is typically < 1 ng/ml. The specific activity was determined by the dose-dependent cytotoxicity of the TNF-alpha sensitive cell line L-929 cells in the presence of the metabolic inhibitor Actinomycin D using BioVision’s Quick Cell Proliferation colorimetric Assay Kit Plus (Catalog # K302).

ED50 is typically < 1 ng/ml

18 kDa with a 6×His tag at the C-terminus

-20°C

Gel Pack

12 months

Lyophilized powder

Lyophilized

Centrifuge the vial prior to opening.

The TNF-alpha Extracellular Domain, Recombinant is a α- or alpha protein sometimes glycoprotein present in blood.

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Recombinants or rec. proteins

recombinants

Tumor necrosis factor (TNFa, tumor necrosis factor alpha, TNFα, cachexin, or cachectin) is a cell signaling protein (cytokine) involved in systemic inflammation and is one of the cytokines that make up the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. TNFb or TNF beta also bin on TNF receptors for Th1 activation.