Human CellExp Pro-IGF-II, Human Recombinant

Catalog number
6477-50
Name
Human CellExp Pro-IGF-II, Human Recombinant
Size
50 µg
Price
1195.00 EUR
Supplier
Biovision
Order

Details

Synonyms
Human Cellexp Human Recombinant Pro-IGF-II
Alternates names
Pro-insln like Growth Factor-2, pro-IGF2.
Taglines
A growth promoting hormone during gestation
Biomolecule/Target
NA
NCBI Gene ID #.
3481
NCBI Gene Symbol
IGF2
Gene Source
Human
Accession #
P01344
Recombinant
Yes
Source
Human 293 cell expressed
Purity
N/A
Endotoxin Level
< 1.0 EU per 1 μg of protein
Activity (Specifications/test method)
N/A
Biological activity
ED₅₀ is typically 2 to 8 ng/mL. The specific activity was determined by the dose-dependent stimulation of the proliferation of MCF-7 cells (human breast cancer cell line).
Results
2 to 8 ng/ml
Molecular Weight
25 kDa, monomer, glycosylated
Storage Temp.
-20°C
Shipping
gel pack
Shelf Life
1 year
Concentration
N/A
Appearance
Lyophilized
Physical form description
Lyophilized from a solution of 50 mM sodium acetate, pH 4.5, with 350 mM NaCl.
Reconstitution Instructions
Reconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.
Handling
Centrifuge the vial prior to opening.
Usage
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Description
IGF-I, IGF-II, insulin-like growth factors -1 and -2 are important regulators of signaling that mediates growth and metabolism in cells. Insulin-like growth factor -1 shares structural homology with proinsulin and is mainly produced by liver. IGF-1 acts via specific receptor, IGF-1R, a heterodimeric tyrosine kinase receptor that signals to various second messenger pathways such as MAPK, Raf/Ras and PI3K cascades.
Additional source
Recombinants or rec. proteins
Group
recombinants