Human CellExp DKK-1, Human Recombinant

Catalog number
7132-10
Name
Human CellExp DKK-1, Human Recombinant
Size
10 μg
Price
360.00 EUR
Supplier

Details

Synonyms
Dickkopf-related protein-1, Dickkopf-1, SK
Alternates names
Dickkopf-related protein-1, Dickkopf-1, SK
Taglines
Plays an important regulatory role in the Wnt /β-catenin signaling pathway
Biomolecule/Target
DKK-1
NCBI Gene ID #.
22943
NCBI Gene Symbol
DKK-1
Gene Source
Human
Accession #
O94907
Recombinant
Yes
Source
HEK 293 cells
Purity
N/A
Endotoxin Level
< 0.1 ng/μg of protein (
Activity (Specifications/test method)
Determined by its ability to inhibit the proliferation of HCT116 colorectal carcinoma cells. Approximately 40% growth inhibition was achieved at a DKK-1 concentration of 200 ng/ml.
Biological activity
Determined by its ability to inhibit the proliferation of HCT116 colorectal carcinoma cells. Approximately 40% growth inhibition was achieved at a DKK-1 concentration of 200 ng/ml.
Results
N/A
Molecular Weight
35-40 kDa
Storage Temp.
-20°C
Shipping
Gel Pack
Shelf Life
1 year
Concentration
N/A
Appearance
Lyophilized powder
Physical form description
Sterile filtered through a 0.2 micron filter. Lyophilized from 1X PBS, pH 7.5.
Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Handling
Centrifuge the vial prior to opening.
Usage
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants