Human CellExp DKK-1, Human Recombinant

Catalog number

7132-50

Size

50 μg

Price

1145.00 EUR

Supplier

Biovision

Order

Details

Synonyms

Dickkopf-related protein-1, Dickkopf-1, SK

Alternates names

Dickkopf-related protein-1, Dickkopf-1, SK

Taglines

Plays an important regulatory role in the Wnt /β-catenin signaling pathway

Biomolecule/Target

DKK-1

NCBI Gene ID #.

22943

NCBI Gene Symbol

DKK-1

Gene Source

Human

Accession #

O94907

Recombinant

Yes

Source

HEK 293 cells

Purity

N/A

Endotoxin Level

< 0.1 ng/μg of protein (

Activity (Specifications/test method)

Determined by its ability to inhibit the proliferation of HCT116 colorectal carcinoma cells. Approximately 40% growth inhibition was achieved at a DKK-1 concentration of 200 ng/ml.

Biological activity

Determined by its ability to inhibit the proliferation of HCT116 colorectal carcinoma cells. Approximately 40% growth inhibition was achieved at a DKK-1 concentration of 200 ng/ml.

Results

N/A

Molecular Weight

35-40 kDa

Storage Temp.

-20°C

Shipping

Gel Pack

Shelf Life

1 year

Concentration

N/A

Appearance

Lyophilized powder

Physical form description

Sterile filtered through a 0.2 micron filter. Lyophilized from 1X PBS, pH 7.5.

Reconstitution Instructions

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Handling

Centrifuge the vial prior to opening.

Usage

For Research Use Only! Not to be used in humans

Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Additional source

Recombinants or rec. proteins

Group

recombinants