Human CellExp TLR-3, Human Recombinant
Catalog number
7220-10
Name
Human CellExp TLR-3, Human Recombinant
Size
10 μg
Price
295.00 EUR
Supplier
Details
Synonyms
Toll-like receptor 3; CD283 antigen
Alternates names
Toll-like receptor 3; CD283 antigen
Taglines
Promotes the NF-kB pathway to initiate innate and adaptive immune responses
Biomolecule/Target
TLR3
NCBI Gene ID #.
7098
NCBI Gene Symbol
TLR3
Gene Source
Human
Accession #
O15455
Recombinant
Yes
Source
HEK 293 cells
Purity
N/A
Endotoxin Level
< 0.1 ng/μg of protein (
Activity (Specifications/test method)
Determined by its ability to inhibit IL-8 secretion induced by Poly I:C in the TLR3 receptor-expressing HEK293 cells (HEK293 TLR3r). The ED50 for this effect is ≤ 30 μg/ml in the presence of 100 μg/ml of Poly I:C.
Biological activity
Determined by its ability to inhibit IL-8 secretion induced by Poly I:C in the TLR3 receptor-expressing HEK293 cells (HEK293 TLR3r). The ED50 for this effect is ≤ 30 μg/ml in the presence of 100 μg/ml of Poly I:C.
Results
N/A
Molecular Weight
77.4 kDa
Storage Temp.
-20°C
Shipping
Gel Pack
Shelf Life
1 year
Concentration
N/A
Appearance
Lyophilized powder
Physical form description
Sterile filtered through a 0.2 micron filter. Lyophilized from 20 mM Sodium Phosphate, pH 7.5.
Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Handling
Centrifuge the vial prior to opening.
Usage
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants
