Human CellExp SHH, human recombinant

Catalog number

7223-10

Size

10 μg

Price

300.00 EUR

Supplier

Biovision

Order

Details

Synonyms

SHH, HHG-1, Sonic hedgehog, HLP3, HPE3, SMMCI, TPT, TPTPS

Alternates names

SHH, HHG-1, Sonic hedgehog, HLP3, HPE3, SMMCI, TPT, TPTPS

Taglines

Hedgehog signaling pathway ligand involved in regulating vertebrate organogenesis

Biomolecule/Target

SHH

NCBI Gene ID #.

6469

NCBI Gene Symbol

SHH

Gene Source

Human

Accession #

Q15465

Recombinant

Yes

Source

HEK 293 cells

Purity

N/A

Endotoxin Level

< 0.1 ng/μg of protein (

Activity (Specifications/test method)

Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is typically 1.5-10 μg/mL.

Biological activity

Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is typically 1.5-10 μg/mL.

Results

N/A

Molecular Weight

This protein is fused with 6 × His tag at the C-terminus, has a calculated MW of 20.4 kDa. The predicted N-terminus is Cys 24. DTT-reduced protein migrates as 22 kDa.

Storage Temp.

-20°C

Shipping

Gel Pack

Shelf Life

1 year

Concentration

N/A

Appearance

Lyophilized powder

Physical form description

Lyophilized from 0.22 μm filtered solution in PBS. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Reconstitution Instructions

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Handling

Centrifuge the vial prior to opening.

Usage

For Research Use Only! Not to be used in humans

Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Additional source

Recombinants or rec. proteins

Group

recombinants