Human CellExp IL-17E, human recombinant

Catalog number
7224-10
Name
Human CellExp IL-17E, human recombinant
Size
10 μg
Price
445.00 EUR
Supplier
Biovision
Order

Details

Synonyms
IL-17E, IL-25, Interleukin-25
Alternates names
IL-17E, IL-25, Interleukin-25
Taglines
Induces NF-κB activation and stimulates the production of IL8
Biomolecule/Target
IL-17E
NCBI Gene ID #.
64806
NCBI Gene Symbol
IL-17E
Gene Source
Human
Accession #
Q9H293
Recombinant
Yes
Source
HEK 293 cells
Purity
N/A
Endotoxin Level
< 0.1 ng/μg of protein (
Activity (Specifications/test method)
The activity is determined by the dose-dependent production of IL-8 by human PBMCs. The expected ED50 for this effect is 12-25 ng/ml.
Biological activity
The activity is determined by the dose-dependent production of IL-8 by human PBMCs. The expected ED50 for this effect is 12-25 ng/ml.
Results
N/A
Molecular Weight
This protein is fused with 6 × His tag at the C-terminus, has a calculated MW of 16.7 kDa. The predicted N-terminus is Tyr 33. DTT-reduced Protein migrates as 24 kDa, 27 kDa and 31 kDa due to different glycosylation.
Storage Temp.
-20°C
Shipping
Gel Pack
Shelf Life
1 year
Concentration
N/A
Appearance
Lyophilized powder
Physical form description
Lyophilized from 0.22 μm filtered solution in PBS. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Handling
Centrifuge the vial prior to opening.
Usage
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants