Human CellExp EGF, human recombinant

Catalog number

7228-10

Size

10 μg

Price

360.00 EUR

Supplier

Biovision

Order

Details

Synonyms

EGF, URG, HOMG4, epidermal growth factor

Alternates names

EGF, URG, HOMG4, epidermal growth factor

Taglines

Plays an important role in the regulation of cell growth

Biomolecule/Target

EGF

NCBI Gene ID #.

1950

NCBI Gene Symbol

EGF

Gene Source

Human

Accession #

P01133

Recombinant

Yes

Source

HEK 293 cells

Purity

N/A

Endotoxin Level

< 0.1 ng/μg of protein (

Activity (Specifications/test method)

Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 20-100 pg/mL.

Biological activity

Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 20-100 pg/mL.

Results

N/A

Molecular Weight

This protein has a calculated MW of 6.3 kDa. DTT-reduced protein migrates as 6.3 kDa estimated polypeptide in SDS-PAGE.

Storage Temp.

-20°C

Shipping

Gel Pack

Shelf Life

1 year

Concentration

N/A

Appearance

Lyophilized powder

Physical form description

Lyophilized from 0.22 μm filtered solution in PBS. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Reconstitution Instructions

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Handling

Centrifuge the vial prior to opening.

Usage

For Research Use Only! Not to be used in humans

Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Additional source

Recombinants or rec. proteins

Group

recombinants