Human CellExp VEGF R1 /Flt-1, human recombinant
Catalog number
7237-10
Name
Human CellExp VEGF R1 /Flt-1, human recombinant
Size
10 μg
Price
280.00 EUR
Supplier
Details
Synonyms
FLT, VEGFR1, FLT1
Alternates names
FLT, VEGFR1, FLT1
Taglines
Plays an essential role in the development of embryonic vasculature
Biomolecule/Target
VEGFR1
NCBI Gene ID #.
2321
NCBI Gene Symbol
VEGFR1
Gene Source
Human
Accession #
P17948
Recombinant
Yes
Source
HEK 293 cells
Purity
N/A
Endotoxin Level
< 0.1 ng/μg of protein (
Activity (Specifications/test method)
Measured by its ability to inhibit the VEGF-dependent proliferation of HUVEC human umbilical vein endothelial cells. The ED50 for this effect is typically 2-25 ng/ml.
Biological activity
Measured by its ability to inhibit the VEGF-dependent proliferation of HUVEC human umbilical vein endothelial cells. The ED50 for this effect is typically 2-25 ng/ml.
Results
N/A
Molecular Weight
The protein is fused with 6×His tag at the N-terminus, has a calculated MW of 50.6 kDa. The predicted N-terminus is Asp 34. DTT-reduced Protein migrates as 55-60 kDa due to glycosylation.
Storage Temp.
-20°C
Shipping
Gel Pack
Shelf Life
1 year
Concentration
N/A
Appearance
Lyophilized powder
Physical form description
Lyophilized from 0.22 μm filtered solution in PBS. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Handling
Centrifuge the vial prior to opening.
Usage
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants