Human CellExp PLGF/PIGF2/PGF, human recombinant

Catalog number

7238-10

Size

10 μg

Price

535.00 EUR

Supplier

Biovision

Order

Details

Synonyms

PGF, PLGF, PlGF2, PlGF, PGFL, SHGC-10760

Alternates names

PGF, PLGF, PlGF2, PlGF, PGFL, SHGC-10760

Taglines

A key molecule in angiogenesis and vasculogenesis

Biomolecule/Target

PLGF

NCBI Gene ID #.

5228

NCBI Gene Symbol

PLGF

Gene Source

Human

Accession #

P49763

Recombinant

Yes

Source

HEK 293 cells

Purity

N/A

Endotoxin Level

< 0.1 ng/μg of protein (

Activity (Specifications/test method)

Measured in a cell proliferation assay using MDA - MB – 231 human breast cancer cells. The ED50 for this effect is typically 1 - 10 µg/ml.

Biological activity

Measured in a cell proliferation assay using MDA - MB – 231 human breast cancer cells. The ED50 for this effect is typically 1 - 10 µg/ml.

Results

N/A

Molecular Weight

The protein is fused with 6×His tag at the C-terminus, has a calculated MW of 20.2 kDa. The predicted N-terminus is Leu 19. DTT-reduced Protein migrates as 28-33 kDa due to glycosylation.

Storage Temp.

-20°C

Shipping

Gel Pack

Shelf Life

1 year

Concentration

N/A

Appearance

Lyophilized powder

Physical form description

Lyophilized from 0.22 μm filtered solution in PBS. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Reconstitution Instructions

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Handling

Centrifuge the vial prior to opening.

Usage

For Research Use Only! Not to be used in humans

Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Additional source

Recombinants or rec. proteins

Group

recombinants