Human CellExp HMGB1 /HMG1, human recombinant

Catalog number
7240-10
Name
Human CellExp HMGB1 /HMG1, human recombinant
Size
10 μg
Price
305.00 EUR
Supplier

Details

Synonyms
HMGB1, HMG1, HMG3, SBP-1
Alternates names
HMGB1, HMG1, HMG3, SBP-1
Taglines
A DNA vaccine adjuvant and a target for cancer therapy.
Biomolecule/Target
HMGB1
NCBI Gene ID #.
3146
NCBI Gene Symbol
HMGB1
Gene Source
Human
Accession #
P09429
Recombinant
Yes
Source
HEK 293 cells
Purity
N/A
Endotoxin Level
< 0.1 ng/μg of protein (
Activity (Specifications/test method)
Measured by its binding ability in a functional ELISA Immobilized human HMGB at 2 μg/ml (100 μl/well) can bind human AGER. The EC50 of human AGER is 0.22 μg/ml.
Biological activity
Measured by its binding ability in a functional ELISA Immobilized human HMGB at 2 μg/ml (100 μl/well) can bind human AGER. The EC50 of human AGER is 0.22 μg/ml.
Results
N/A
Molecular Weight
The protein is fused with 6×His tag at the C-terminus, has a calculated MW of 25.7 kDa. The predicted N-terminus is Met1. DTT-reduced Protein migrates as 28 kDa and 32 kDa due to different glycosylation.
Storage Temp.
-20°C
Shipping
Gel Pack
Shelf Life
1 year
Concentration
N/A
Appearance
Lyophilized powder
Physical form description
Lyophilized from 0.22 μm filtered solution in PBS. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Handling
Centrifuge the vial prior to opening.
Usage
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants