Human CellExp Renin, mouse recombinant
Catalog number
7241-10
Name
Human CellExp Renin, mouse recombinant
Size
10 μg
Price
300.00 EUR
Supplier
Details
Synonyms
CYMP, CYM, RENNIN, Chymosin
Alternates names
CYMP, CYM, RENNIN, Chymosin
Taglines
Plays an essential role in the elevation of arterial blood pressure
Biomolecule/Target
Renin
NCBI Gene ID #.
19701
NCBI Gene Symbol
REN
Gene Source
Mouse
Accession #
P06281
Recombinant
Yes
Source
HEK 293 cells
Purity
N/A
Endotoxin Level
< 0.1 ng/μg of protein (
Activity (Specifications/test method)
Measured by its ability to cleave the fluorogenic peptide substrate 5FAM/QXL 520. The specific activity is >25 pmol/min/μg.
Biological activity
Measured by its ability to cleave the fluorogenic peptide substrate 5FAM/QXL 520. The specific activity is >25 pmol/min/μg.
Results
N/A
Molecular Weight
The protein is fused with 6×his tag at the C-terminus and has a calculated MW of 43.2 kDa expressed. The predicted N-terminus is Leu24. Protein migrates as 45-55 kDa in reduced SDS-PAGE due to glycosylation.
Storage Temp.
-20°C
Shipping
Gel Pack
Shelf Life
1 year
Concentration
N/A
Appearance
Lyophilized powder
Physical form description
Lyophilized from 0.22 μm filtered solution in PBS. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Handling
Centrifuge the vial prior to opening.
Usage
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin name
Mus musculus
Additional source
Recombinants or rec. proteins
Group
recombinants
