Human CellExp PCSK9, human recombinant
Catalog number
7265-20
Name
Human CellExp PCSK9, human recombinant
Size
20 μg
Price
460.00 EUR
Supplier
Details
Synonyms
PCSK9, FH3, HCHOLA3, LDLCQ1, NARC-1, NARC1, PC9, Proprotein convertase subtilisin/kexin type 9
Alternates names
PCSK9, FH3, HCHOLA3, LDLCQ1, NARC-1, NARC1, PC9, Proprotein convertase subtilisin/kexin type 9
Taglines
Plays a major regulatory role in cholesterol homeostasis
Biomolecule/Target
PCSK9
NCBI Gene ID #.
255738
NCBI Gene Symbol
PCSK9
Gene Source
Human
Accession #
Q8NBP7
Recombinant
Yes
Source
HEK 293 cells
Purity
N/A
Endotoxin Level
< 0.1 ng/μg of protein (
Activity (Specifications/test method)
Measured by its ability to bind with human LDLR in a functional ELISA Immobilized recombinant human PCSK9 at 10 μg/ml (100 μl/well) can bind biotinylated human LDLR. The ED50 for this effect is typically 15 ng/ml.
Biological activity
Measured by its ability to bind with human LDLR in a functional ELISA Immobilized recombinant human PCSK9 at 10 μg/ml (100 μl/well) can bind biotinylated human LDLR. The ED50 for this effect is typically 15 ng/ml.
Results
N/A
Molecular Weight
This protein fused with polyhistidine tag at the C-terminus, has a calculated MW of 75.1 kDa. The predicted N-terminus is Gln 31. DTT-reduced Protein migrates as 20 kDa and 62 kDa due to glycosylation and proteolytic digestion.
Storage Temp.
-20°C
Shipping
Gel Pack
Shelf Life
1 year
Concentration
N/A
Appearance
Lyophilized powder
Physical form description
Sterile filtered through a 0.22 micron filter. Lyophilized from 1 x PBS, pH 7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 100 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Handling
Centrifuge the vial prior to opening.
Usage
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants
