Human CellExp PCSK9, murine recombinant

Catalog number
7266-20
Name
Human CellExp PCSK9, murine recombinant
Size
20 μg
Price
460.00 EUR
Supplier
Biovision
Order

Details

Synonyms
PCSK9, FH3, HCHOLA3, LDLCQ1, NARC-1, NARC1, PC9, Proprotein convertase subtilisin/kexin type 9
Alternates names
PCSK9, FH3, HCHOLA3, LDLCQ1, NARC-1, NARC1, PC9, Proprotein convertase subtilisin/kexin type 9
Taglines
Plays a major regulatory role in cholesterol homeostasis
Biomolecule/Target
PCSK9
NCBI Gene ID #.
100102
NCBI Gene Symbol
PCSK9
Gene Source
Mouse
Accession #
Q80W65
Recombinant
Yes
Source
HEK 293 cells
Purity
N/A
Endotoxin Level
< 0.1 ng/μg of protein (
Activity (Specifications/test method)
Measured by its binding ability in a functional ELISA Immobilized mouse PCSK9 at 10 μg/ml (100 μl/well) can bind biotinylated rhLDLR. The EC50 of biotinylated human LDLR is 0.1 μg/ml.
Biological activity
Measured by its binding ability in a functional ELISA Immobilized mouse PCSK9 at 10 μg/ml (100 μl/well) can bind biotinylated rhLDLR. The EC50 of biotinylated human LDLR is 0.1 μg/ml.
Results
N/A
Molecular Weight
This protein is fused with 6×His tag at the N-terminus, has a calculated MW of 72 kDa. The predicted N-terminus is Gln 35. DTT-reduced Protein migrates as 20 kDa and 64 kDa due to glycosylation.
Storage Temp.
-20°C
Shipping
Gel Pack
Shelf Life
1 year
Concentration
N/A
Appearance
Lyophilized powder
Physical form description
Sterile filtered through a 0.22 micron filter. Lyophilized from 1 x PBS, pH 7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 100 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Handling
Centrifuge the vial prior to opening.
Usage
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants