Human CellExp LIF, human recombinant

Catalog number
7267-10
Name
Human CellExp LIF, human recombinant
Size
10 μg
Price
460.00 EUR
Supplier

Details

Synonyms
LIF, CDF, DIA, HILDA, MLPLI
Alternates names
LIF, CDF, DIA, HILDA, MLPLI
Taglines
Affects cell growth and development
Biomolecule/Target
LIF
NCBI Gene ID #.
3976
NCBI Gene Symbol
LIF
Gene Source
Human
Accession #
P15018
Recombinant
Yes
Source
HEK 293 cells
Purity
N/A
Endotoxin Level
< 0.1 ng/μg of protein (
Activity (Specifications/test method)
Determined by its ability to stimulate the proliferation of human TF-1 cells. The ED50 1X10^7 units/mg.
Biological activity
Determined by its ability to stimulate the proliferation of human TF-1 cells. The ED50 1X10^7 units/mg.
Results
N/A
Molecular Weight
Calculated MW of 19.9 kDa with Gly-Pro at the N- terminus. The predicted N-terminus is Ser 23. DTT-reduced Protein migrates as 35-45 kDa.
Storage Temp.
-20°C
Shipping
Gel Pack
Shelf Life
1 year
Concentration
N/A
Appearance
Lyophilized powder
Physical form description
Sterile filtered through a 0.22 micron filter. Lyophilized from 1 x PBS, pH 7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in sterile deionized water to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Handling
Centrifuge the vial prior to opening.
Usage
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants