Human CellExp IFN-gamma, human recombinant
Catalog number
7271-10
Name
Human CellExp IFN-gamma, human recombinant
Size
10 μg
Price
340.00 EUR
Supplier
Details
Synonyms
IFNG, IFG, IFI
Alternates names
IFNG, IFG, IFI
Taglines
A central regulator of the immune response and signals
Biomolecule/Target
IFN-gamma
NCBI Gene ID #.
3458
NCBI Gene Symbol
IFN-gamma
Gene Source
Human
Accession #
P01579
Recombinant
Yes
Source
HEK 293 cells
Purity
N/A
Endotoxin Level
< 0.1 ng/μg of protein (
Activity (Specifications/test method)
Determined by a cytotoxicity assay using HT-29 cells. ED50 2X10^7 Unit/mg.
Biological activity
Determined by a cytotoxicity assay using HT-29 cells. ED50 2X10^7 Unit/mg.
Results
N/A
Molecular Weight
Calculated MW of 16.8 kDa with no tag. The predicted N-terminus is Gln 24. DTT-reduced protein migrates as 25.0 kDa due to glycosylation.
Storage Temp.
-20°C
Shipping
Gel Pack
Shelf Life
1 year
Concentration
N/A
Appearance
Lyophilized powder
Physical form description
Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 100 mM NaCl, pH 8.0. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in sterile deionized water to a concentration of 100 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Handling
Centrifuge the vial prior to opening.
Usage
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants
