Human CellExp PDGF-BB, human recombinant

Catalog number

7276-10

Size

10 μg

Price

490.00 EUR

Supplier

Biovision

Order

Details

Synonyms

PDGFB, PDGF-B, FLJ12858, PDGF2, PDGF-2

Alternates names

PDGFB, PDGF-B, FLJ12858, PDGF2, PDGF-2

Taglines

Mitogenic during early developmental stages

Biomolecule/Target

PDGFB

NCBI Gene ID #.

5155

NCBI Gene Symbol

PDGFB

Gene Source

Human

Accession #

P01127

Recombinant

Yes

Source

HEK 293 cells

Purity

N/A

Endotoxin Level

< 0.1 ng/μg of protein (

Activity (Specifications/test method)

The bio-activity was determined by dose-dependent stimulation of the proliferation of mouse 3T3 cells. ED50 was 1-10 ng/ml

Biological activity

The bio-activity was determined by dose-dependent stimulation of the proliferation of mouse 3T3 cells. ED50 was 1-10 ng/ml

Results

N/A

Molecular Weight

This protein has a calculated MW of 25 kDa. DTT-reduced protein migrates as a 15 kDa polypeptide and the non-reduced cystine-linked homodimer migrates as a 30 kDa protein.

Storage Temp.

-20°C

Shipping

Gel Pack

Shelf Life

1 year

Concentration

N/A

Appearance

Lyophilized powder

Physical form description

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl pH 8.0. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Reconstitution Instructions

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Handling

Centrifuge the vial prior to opening.

Usage

For Research Use Only! Not to be used in humans

Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Additional source

Recombinants or rec. proteins

Group

recombinants