Human CellExp ErbB4/HER4, human recombinant
Catalog number
7401-50
Name
Human CellExp ErbB4/HER4, human recombinant
Size
50 μg
Price
745.00 EUR
Supplier
Details
Synonyms
HER4, ErbB4, MGC138404, p180erbB4
Alternates names
HER4, ErbB4, MGC138404, p180erbB4
Taglines
Involved in a variety of cellular responses including mitogenesis and differentiation
Biomolecule/Target
ErbB4
NCBI Gene ID #.
2066
NCBI Gene Symbol
ErbB4
Gene Source
Human
Accession #
Q15303
Recombinant
Yes
Source
HEK293 cells
Purity
N/A
Activity (Specifications/test method)
N/A
Biological activity
Measured by its ability to inhibit the biological activity of Neuregulin-1-β1 on MCF-7 human breast cancer cells, in the presence of 10 ng/mL of Recombinant Human NRG1-β1/HRG1-β1 Extracellular Domain. The ED50 for this effect is typically 0.2-2.5 μg/mL.
Results
Measured by its ability to inhibit the biological activity of Neuregulin-1-β1 on MCF-7 human breast cancer cells, in the presence of 10 ng/mL of Recombinant Human NRG1-β1/HRG1-β1 Extracellular Domain. The ED50 for this effect is typically 0.2-2.5 μg/mL.
Molecular Weight
This protein is fused with 6×his tag at the C-terminus and has a calculated MW of 70.6 kDa expressed. The predicted N-terminal is Glu26. Protein migrates as 95-110 kDa in reduced SDS-PAGE due to glycosylation.
Storage Temp.
-20°C
Shipping
Gel Pack
Shelf Life
1 year
Concentration
N/A
Appearance
Lyophilized
Physical form description
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Handling
Centrifuge the vial prior to opening.
Usage
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants
