Human CellExp ErbB1/EGFR, mouse recombinant
Catalog number
7402-10
Name
Human CellExp ErbB1/EGFR, mouse recombinant
Size
10 μg
Price
380.00 EUR
Supplier
Details
Synonyms
EGFR, ERBB, ERBB1, HER1, PIG61, mENA
Alternates names
EGFR, ERBB, ERBB1, HER1, PIG61, mENA
Taglines
A cell-surface receptor for members of the EGF-family of extracellular protein ligands.
Biomolecule/Target
ErbB1
NCBI Gene ID #.
13649
NCBI Gene Symbol
EGFR
Gene Source
Mouse
Accession #
Q01279
Recombinant
Yes
Source
HEK293 cells
Purity
N/A
Activity (Specifications/test method)
N/A
Biological activity
Measured by its binding ability in a functional ELISA. Immobilized mouse EGF at 10 μg/ml can bind mouse EGFR with a linear range of 2.5–1000 ng/ml.
Results
Measured by its binding ability in a functional ELISA. Immobilized mouse EGF at 10 μg/ml can bind mouse EGFR with a linear range of 2.5–1000 ng/ml.
Molecular Weight
This protein has a C-terminal 6×His tag and has a calculated MW of 70 kDa. The predicted N-terminus is Leu 25. In DTT-reduced SDS-PAGE, protein migrates as 110-115 kDa.
Storage Temp.
-20°C
Shipping
Gel Pack
Shelf Life
1 year
Concentration
N/A
Appearance
Lyophilized
Physical form description
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Handling
Centrifuge the vial prior to opening.
Usage
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin name
Mus musculus
Additional source
Recombinants or rec. proteins
Group
recombinants
