Human CellExp ApoA-1, human recombinant

Catalog number
7403-10
Name
Human CellExp ApoA-1, human recombinant
Size
10 μg
Price
370.00 EUR
Supplier
Biovision
Order

Details

Synonyms
Apolipoprotein A-I, APOA1, MGC117399
Alternates names
Apolipoprotein A-I, APOA1, MGC117399
Taglines
Promotes cholesterol efflux from tissues to the liver for excretion.
Biomolecule/Target
ApoA1
NCBI Gene ID #.
335
NCBI Gene Symbol
ApoA1
Gene Source
Human
Accession #
P02647
Recombinant
Yes
Source
HEK293 cells
Purity
N/A
Activity (Specifications/test method)
N/A
Biological activity
Immobilized Human ApoAI at 10 μg/mL (100 μl/well) can bind biotinylated human SCARB1. The EC50 of biotinylated human SCARB1 is 10-100 ng/mL.
Results
Immobilized Human ApoAI at 10 μg/mL (100 μl/well) can bind biotinylated human SCARB1. The EC50 of biotinylated human SCARB1 is 10-100 ng/mL.
Molecular Weight
This protein is fused with polyhistidine tag at the C-terminus, and has a calculated MW of 29 kDa. The predicted N-terminus is Asp 25. DTT-reduced Protein migrates as 26-30 kDa in SDS-PAGE.
Storage Temp.
-20°C
Shipping
Gel Pack
Shelf Life
1 year
Concentration
N/A
Appearance
Lyophilized
Physical form description
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in sterile deionized water to a concentration of 50 µg/ml. Solubilize for 30 to 60 min. at RT with occasional gentle mixing. Do not vortex. Carrier protein (0.1% HAS or BSA) is strongly recommended for further dilution and long term storage.
Handling
Centrifuge the vial prior to opening.
Usage
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants