Human CellExp Cathepsin B, human recombinant
Catalog number
7408-10
Name
Human CellExp Cathepsin B, human recombinant
Size
10 μg
Price
370.00 EUR
Supplier
Details
Synonyms
CTSB, CPSB, APPS
Alternates names
CTSB, CPSB, APPS
Taglines
Participates in intracellular degradation and turnover of proteins
Biomolecule/Target
Cathepsin B
NCBI Gene ID #.
1508
NCBI Gene Symbol
CTSB
Gene Source
Human
Accession #
P07858
Recombinant
Yes
Source
HEK293 cells
Purity
N/A
Activity (Specifications/test method)
N/A
Biological activity
Measured by its ability to cleave the fluorogenic peptide substrate Z-LR-AMC. Measured in 100 μl reaction mixture containing 25 mM MES, pH 5.0, 0.01 µg rhCathepsin B, and 10 µM reaction substrate. The specific activity is >2800 pmol/min/µg.
Results
Measured by its ability to cleave the fluorogenic peptide substrate Z-LR-AMC. Measured in 100 μl reaction mixture containing 25 mM MES, pH 5.0, 0.01 µg rhCathepsin B, and 10 µM reaction substrate. The specific activity is >2800 pmol/min/µg.
Molecular Weight
This protein is fused with a 6×His tag at the C-terminus, has a calculated MW of 36.7 kDa (Pro) and 29 kDa (Mature). The predicted N-terminus is Arg18 (pro) or Phe74 (mature). DTT-reduced Protein migrates as 43 kDa and 34 kDa due to glycosylation.
Storage Temp.
-20°C
Shipping
Gel Pack
Shelf Life
1 year
Concentration
N/A
Appearance
Lyophilized
Physical form description
Lyophilized from 0.22 μm filtered solution in 50 mM Tris-HCl and 150 mM NaCl, pH 8.0. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Handling
Centrifuge the vial prior to opening.
Usage
For Research Use Only! Not to be used in humans
Gene
CTS protease activity also measured by zymograph electrophoresis of Cathepsins. This cathepsin is supplied in 1.
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants
