Human CellExp Cathepsin L1, human recombinant

Catalog number
7410-10
Name
Human CellExp Cathepsin L1, human recombinant
Size
10 μg
Price
380.00 EUR
Supplier
Biovision
Order

Details

Synonyms
CTSL1, MEP, CATL, CTSL
Alternates names
CTSL1, MEP, CATL, CTSL
Taglines
Plays a major role in intracellular protein catabolism.
Biomolecule/Target
Cathepsin L1
NCBI Gene ID #.
1514
NCBI Gene Symbol
CTSL
Gene Source
Human
Accession #
P07711
Recombinant
Yes
Source
HEK293 cells
Purity
N/A
Activity (Specifications/test method)
N/A
Biological activity
Measured by its binding ability in a functional ELISA. Immobilized human CD74 at 5 μg/ml (100 μl/well) can bind biotinylated human CTSL1 with a linear range of 25 - 400 ng/ml.
Results
Measured by its binding ability in a functional ELISA. Immobilized human CD74 at 5 μg/ml (100 μl/well) can bind biotinylated human CTSL1 with a linear range of 25 - 400 ng/ml.
Molecular Weight
This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 36.7 kDa. The predicted N-terminus is Thr 18 and Ala 114. DTT-reduced Protein migrates as 10 kDa and 34 kDa due to glycosylation, corresponding to the propeptide and the mature form respectively.
Storage Temp.
-20°C
Shipping
Gel Pack
Shelf Life
1 year
Concentration
N/A
Appearance
Lyophilized
Physical form description
Lyophilized from 0.22 μm filtered solution in 50 mM Sodium Acetate, pH 5.0 with 100 mM NaCl. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Handling
Centrifuge the vial prior to opening.
Usage
For Research Use Only! Not to be used in humans
Gene
CTS protease activity also measured by zymograph electrophoresis of Cathepsins. This cathepsin is supplied in 1.
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants