Human CellExp SERPIN A3, human recombinant

Catalog number

7419-10

Size

10 μg

Price

370.00 EUR

Supplier

Biovision

Order

Details

Synonyms

SERPINA3, ACT, AACT, GIG24, GIG25

Alternates names

SERPINA3, ACT, AACT, GIG24, GIG25

Taglines

Involved in inhibition of neutrophil cathepsin G and mast cell chymase

Biomolecule/Target

SERPIN A3

NCBI Gene ID #.

NCBI Gene Symbol

SERPINA3

Gene Source

Human

Accession #

P01011

Recombinant

Yes

Source

HEK293 cells

Purity

N/A

Activity (Specifications/test method)

N/A

Biological activity

Measured by its ability to inhibit chymotrypsin cleavage of a fluorogenic peptide substrate MCA-RPKPVE-Nval-WRK (Dnp)-NH2. The IC50 value is

Results

Measured by its ability to inhibit chymotrypsin cleavage of a fluorogenic peptide substrate MCA-RPKPVE-Nval-WRK (Dnp)-NH2. The IC50 value is

Molecular Weight

This protein is fused with polyhistidine tag at the C-terminus, has a calculated MW of 46 kDa. The predicted N-terminus is His 23. DTT-reduced Protein migrates as 55-70 kDa due to glycosylation.

Storage Temp.

-20°C

Shipping

Gel Pack

Shelf Life

1 year

Concentration

N/A

Appearance

Lyophilized

Physical form description

Lyophilized from 0.22 μm filtered solution in 20 mM HEPES, 150 mM NaCl, pH 7.5. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Reconstitution Instructions

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Handling

Centrifuge the vial prior to opening.

Usage

For Research Use Only! Not to be used in humans

Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Additional source

Recombinants or rec. proteins

Group

recombinants