Human CellExp Fas Ligand/FasL, human recombinant
Catalog number
7443-10
Name
Human CellExp Fas Ligand/FasL, human recombinant
Size
10 μg
Price
485.00 EUR
Supplier
Details
Synonyms
FASLG, ALPS1B, APT1LG1, CD178, CD95-L, CD95L, FASL, TNFSF6, Fas ligand
Alternates names
FASLG, ALPS1B, APT1LG1, CD178, CD95-L, CD95L, FASL, TNFSF6, Fas ligand
Taglines
Fas ligand/receptor interactions play an important role in the regulation of the immune system and the progression of cancer.
Biomolecule/Target
Fas Ligand/FasL
NCBI Gene ID #.
356
NCBI Gene Symbol
FASLG
Gene Source
Human
Accession #
P48023
Recombinant
Yes
Source
HEK293 cells
Purity
N/A
Activity (Specifications/test method)
N/A
Biological activity
Measured by its ability to induce apoptosis of Jurkat human acute T cell leukemia cells. The ED50 for this effect is typically 0.1-1.5 ng/mL in the presence of 10 μg/mL of a crosslinking antibody Mouse Anti poly-Histidine Monoclonal Antibody.
Results
Measured by its ability to induce apoptosis of Jurkat human acute T cell leukemia cells. The ED50 for this effect is typically 0.1-1.5 ng/mL in the presence of 10 μg/mL of a crosslinking antibody Mouse Anti poly-Histidine Monoclonal Antibody.
Molecular Weight
This protein is fused with 6×His tag at N-terminus, has a calculated MW of 17.7 kDa. The predicted N-terminus is His. Protein migrates as 25-32 kDa in reduced SDS-PAGE due to glycosylation.
Storage Temp.
-20°C
Shipping
Gel Pack
Shelf Life
1 year
Concentration
N/A
Appearance
Lyophilized
Physical form description
Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH 7.0. Normally Mannitol or Trehalose is added as protectants before lyophilization.
Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Handling
Centrifuge the vial prior to opening.
Usage
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Description
FAS ligand and other ligands are binding to the receptor for signaling pathways for example in apoptosis or JNK signaling. Receptor agonists are often tested for drug development.
Additional source
Recombinants or rec. proteins
Group
recombinants
