Human CellExp Neprilysin /CD10, human recombinant

Catalog number

7445-10

Size

10 μg

Price

500.00 EUR

Supplier

Biovision

Order

Details

Synonyms

MME, CALLA, CD10, DKFZp686O16152, MGC126681, MGC126707, NEP, SFE, Neprilysin

Alternates names

MME, CALLA, CD10, DKFZp686O16152, MGC126681, MGC126707, NEP, SFE, Neprilysin

Taglines

The rate-limiting degrading enzyme of amyloid β peptide (Aβ)

Biomolecule/Target

Neprilysin /CD10

NCBI Gene ID #.

4311

NCBI Gene Symbol

MME

Gene Source

Human

Accession #

P08473

Recombinant

Yes

Source

HEK293 cells

Purity

N/A

Activity (Specifications/test method)

N/A

Biological activity

Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK-(Dnp)-OH. The specific activity is >2500 pmol/min/μg.

Results

Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK-(Dnp)-OH. The specific activity is >2500 pmol/min/μg.

Molecular Weight

This protein with Gly-Pro at the N- terminus, has a calculated MW of 80 kDa. The predicted N-terminus is Tyr 52. DTT-reduced Protein migrates as 90-100 kDa due to glycosylation.

Storage Temp.

-20°C

Shipping

Gel Pack

Shelf Life

1 year

Concentration

N/A

Appearance

Lyophilized

Physical form description

Lyophilized from 0.22 μm filtered solution in 50 mM tris, 150 mM NaCl, pH 8.0 Normally Mannitol or Trehalose is added as protectants before lyophilization.

Reconstitution Instructions

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Handling

Centrifuge the vial prior to opening.

Usage

For Research Use Only! Not to be used in humans

Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Additional source

Recombinants or rec. proteins

Group

recombinants