Human CellExp EpCAM/CD326, human recombinant

Catalog number
7450-10
Name
Human CellExp EpCAM/CD326, human recombinant
Size
10 μg
Price
330.00 EUR
Supplier
Biovision
Order

Details

Synonyms
EPCAM, DIAR5, DIAR-5, EGP-2, EGP2, EGP314, EGP-314, EGP40, EGP-40, ESA, GA733-2, HNPCC8, HNPCC-8, KS1/4, KSA, M4S1, MIC18, MIC-18, MK-1, MK1, TACSTD1, TACSTD-1, TROP1, TROP-1, CD326, CD-326, Epithelial cell adhesion molecule, tumor-associated calcium signal transducer 1, cluster of differentiation 326.
Alternates names
EPCAM, DIAR5, DIAR-5, EGP-2, EGP2, EGP314, EGP-314, EGP40, EGP-40, ESA, GA733-2, HNPCC8, HNPCC-8, KS1/4, KSA, M4S1, MIC18, MIC-18, MK-1, MK1, TACSTD1, TACSTD-1, TROP1, TROP-1, CD326, CD-326, Epithelial cell adhesion molecule, tumor-associated calcium signal transducer 1, cluster of differentiation 326.
Taglines
A pan-epithelial differentiation antigen that is expressed on almost all carcinomas
Biomolecule/Target
EpCAM/CD326
NCBI Gene ID #.
4072
NCBI Gene Symbol
EPCAM
Gene Source
Human
Accession #
P16422
Recombinant
Yes
Source
HEK293 cells
Purity
N/A
Activity (Specifications/test method)
N/A
Biological activity
Measured by its binding ability in a functional ELISA. Immobilized recombinant human Cathepsin V at 10 μg/mL (100 μl/well) can bind biotinylated EpCAM. The EC50 of biotinylated EpCAM is 50-70 ng/mL.
Results
Measured by its binding ability in a functional ELISA. Immobilized recombinant human Cathepsin V at 10 μg/mL (100 μl/well) can bind biotinylated EpCAM. The EC50 of biotinylated EpCAM is 50-70 ng/mL.
Molecular Weight
This protein contains a poly histidine tag at C-terminus and has a calculated MW of 29 kDa after removal of signal peptide. The predicted N-terminus is Gln24. In DTT-reduced SDS-PAGE, rh EpCAM protein migrates as 33-36 kDa poly peptide due to glycosylation.
Storage Temp.
-20°C
Shipping
Gel Pack
Shelf Life
1 year
Concentration
N/A
Appearance
Lyophilized
Physical form description
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Handling
Centrifuge the vial prior to opening.
Usage
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants