Human CellExp ApoJ/Clusterin, human recombinant

Catalog number
7459-10
Name
Human CellExp ApoJ/Clusterin, human recombinant
Size
10 μg
Price
365.00 EUR
Supplier

Details

Synonyms
TRPM-2, Apolipoprotein J, APO-J, CLI, CLU, SGP-2, AAG4, KUB1, SGP2, SP-40, TRPM2, MGC24903.
Alternates names
TRPM-2, Apolipoprotein J, APO-J, CLI, CLU, SGP-2, AAG4, KUB1, SGP2, SP-40, TRPM2, MGC24903.
Taglines
A heavily glycosylated protein upregulated in multiple pathological conditions
Biomolecule/Target
ApoJ/Clusterin
NCBI Gene ID #.
1191
NCBI Gene Symbol
CLU
Gene Source
Human
Accession #
P10909
Recombinant
Yes
Source
HEK293 cells
Purity
N/A
Activity (Specifications/test method)
N/A
Biological activity
Measured by its ability to induce clustering of human clear cell carcinoma epithelial cells Caki-2. Measured by its ability to inhibit DTT (Dithiothreitol) induced BSA precipitation.
Results
Measured by its ability to induce clustering of human clear cell carcinoma epithelial cells Caki-2. Measured by its ability to inhibit DTT (Dithiothreitol) induced BSA precipitation.
Molecular Weight
This protein is fused is fused with 6 ×his tag at the C terminus, and has a calculated MW of 50.9 kDa (Asp23-Glu449, α chain 24.5kDa + β chain 27kDa). The recombinant rh CLUS / Clusterin (Asp23-Glu 449) was cleaved into α chain and β chain, which form a heterodimer linked by disulfide bonds. DTT reduced protein migrates as 34-37kDa bands in SDS-PAGE due to glycosylation, corresponding to the cleaved β chain, and α chain respectively.
Storage Temp.
-20°C
Shipping
Gel Pack
Shelf Life
1 year
Concentration
N/A
Appearance
Lyophilized
Physical form description
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Handling
Centrifuge the vial prior to opening.
Usage
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants