Human CellExp ApoJ/Clusterin, human recombinant

Catalog number

7459-50

Size

50 μg

Price

795.00 EUR

Supplier

Biovision

Order

Details

Synonyms

TRPM-2, Apolipoprotein J, APO-J, CLI, CLU, SGP-2, AAG4, KUB1, SGP2, SP-40, TRPM2, MGC24903.

Alternates names

TRPM-2, Apolipoprotein J, APO-J, CLI, CLU, SGP-2, AAG4, KUB1, SGP2, SP-40, TRPM2, MGC24903.

Taglines

A heavily glycosylated protein upregulated in multiple pathological conditions

Biomolecule/Target

ApoJ/Clusterin

NCBI Gene ID #.

1191

NCBI Gene Symbol

CLU

Gene Source

Human

Accession #

P10909

Recombinant

Yes

Source

HEK293 cells

Purity

N/A

Activity (Specifications/test method)

N/A

Biological activity

Measured by its ability to induce clustering of human clear cell carcinoma epithelial cells Caki-2. Measured by its ability to inhibit DTT (Dithiothreitol) induced BSA precipitation.

Results

Measured by its ability to induce clustering of human clear cell carcinoma epithelial cells Caki-2. Measured by its ability to inhibit DTT (Dithiothreitol) induced BSA precipitation.

Molecular Weight

This protein is fused is fused with 6 ×his tag at the C terminus, and has a calculated MW of 50.9 kDa (Asp23-Glu449, α chain 24.5kDa + β chain 27kDa). The recombinant rh CLUS / Clusterin (Asp23-Glu 449) was cleaved into α chain and β chain, which form a heterodimer linked by disulfide bonds. DTT reduced protein migrates as 34-37kDa bands in SDS-PAGE due to glycosylation, corresponding to the cleaved β chain, and α chain respectively.

Storage Temp.

-20°C

Shipping

Gel Pack

Shelf Life

1 year

Concentration

N/A

Appearance

Lyophilized

Physical form description

Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Reconstitution Instructions

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Handling

Centrifuge the vial prior to opening.

Usage

For Research Use Only! Not to be used in humans

Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Additional source

Recombinants or rec. proteins

Group

recombinants