Human CellExp R-Spondin 3 /RSPO3 (22-146), human recombinant
Catalog number
7463-10
Name
Human CellExp R-Spondin 3 /RSPO3 (22-146), human recombinant
Size
10 μg
Price
345.00 EUR
Supplier
Details
Synonyms
RSPO3, CRISTIN1, PWTSR, THSD2
Alternates names
RSPO3, CRISTIN1, PWTSR, THSD2
Taglines
Activator of the beta-catenin signaling cascade
Biomolecule/Target
R-Spondin 3 /RSPO3 (22-146)
NCBI Gene ID #.
84870
NCBI Gene Symbol
RSPO3
Gene Source
Human
Accession #
Q9BXY4
Recombinant
Yes
Source
HEK293 cells
Purity
N/A
Activity (Specifications/test method)
N/A
Biological activity
Measured by its ability to induce activation of beta -catenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED50 for this effect is typically 0.5 - 2.0 ng/ml in the presence of 5 ng/mL rmWnt-3a.
Results
Measured by its ability to induce activation of beta -catenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED50 for this effect is typically 0.5 - 2.0 ng/ml in the presence of 5 ng/mL rmWnt-3a.
Molecular Weight
This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 15.3 kDa. The predicted N-terminus is Gln 22. DTT-reduced Protein migrates as 18 kDa and 24 kDa due to different glycosylation.
Storage Temp.
-20°C
Shipping
Gel Pack
Shelf Life
1 year
Concentration
N/A
Appearance
Lyophilized
Physical form description
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Handling
Centrifuge the vial prior to opening.
Usage
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants
