Human CellExp LDLR, human recombinant

Catalog number
7472-10
Name
Human CellExp LDLR, human recombinant
Size
10 μg
Price
380.00 EUR
Supplier

Details

Synonyms
LDLR, FH, FHC, LDLCQ2, Low-Density Lipoprotein (LDL) Receptor
Alternates names
LDLR, FH, FHC, LDLCQ2, Low-Density Lipoprotein (LDL) Receptor
Taglines
Mediates the endocytosis of cholesterol-rich LDL
Biomolecule/Target
LDLR
NCBI Gene ID #.
3949
NCBI Gene Symbol
LDLR
Gene Source
Human
Accession #
P01130
Recombinant
Yes
Source
HEK293 cells
Purity
N/A
Activity (Specifications/test method)
N/A
Biological activity
Measured in a competitive binding assay. When human LDL is immobilized at 1 μg/ml (100 μl/well), recombinant human LDLR inhibits 50% binding of biotinylated recombinant human LDLR (0.5 μg/ml) at the concentration range of 0.35 – 2.5 μg/ml.
Results
Measured in a competitive binding assay. When human LDL is immobilized at 1 μg/ml (100 μl/well), recombinant human LDLR inhibits 50% binding of biotinylated recombinant human LDLR (0.5 μg/ml) at the concentration range of 0.35 – 2.5 μg/ml.
Molecular Weight
This protein is fused with a C-terminal 6×his tag and has a calculated MW of 86 kDa. The predicted N-terminal is Ala22 or Asp193. Corresponding to the mature and immature form, DTT-reduced protein migrates as 95-110 kDa &125-140 kDa polypeptide in SDS-PAGE resulting from different glycosylation.
Storage Temp.
-20°C
Shipping
Gel Pack
Shelf Life
1 year
Concentration
N/A
Appearance
Lyophilized
Physical form description
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Handling
Centrifuge the vial prior to opening.
Usage
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants