Human CellExp CTLA4/CD152, human recombinant

CTLA4, CD152, CELIAC3, GRD4, GSE, ICOS, IDDM12

CTLA4, CD152, CELIAC3, GRD4, GSE, ICOS, IDDM12

A protein receptor that downregulates the immune system.

CTLA4/CD152

1493

CTLA4

Human

P16410

Yes

HEK293 cells

N/A

N/A

Measured by its binding ability in a functional ELISA. Immobilized Human CTLA4 Protein (Catalog # CT4-H5229) at 2 μg/ml (100 μl/well) can bind rhB7-1/ CD80 Protein Fc Chimera (Catalog # B71-H5259) with a linear range of 0.2 - 20 ng/ml, when detected by HRP labeled Goat anti Human IgG Fc PAb. Measured by its binding ability in a functional ELISA. Immobilized Human CTLA4 Protein (Catalog # CT4-H5229) at 1 μg/ml (100 μl/well) can bind rh PD-L1 /B7-H1 /CD274 Protein Fc Chimera (Catalog # PD1-H5258) with a linear range of 20 - 200 ng/ml, when detected by HRP labeled Goat anti Human IgG Fc PAb.

Measured by its binding ability in a functional ELISA. Immobilized Human CTLA4 Protein (Catalog # CT4-H5229) at 2 μg/ml (100 μl/well) can bind rhB7-1/ CD80 Protein Fc Chimera (Catalog # B71-H5259) with a linear range of 0.2 - 20 ng/ml, when detected by HRP labeled Goat anti Human IgG Fc PAb. Measured by its binding ability in a functional ELISA. Immobilized Human CTLA4 Protein (Catalog # CT4-H5229) at 1 μg/ml (100 μl/well) can bind rh PD-L1 /B7-H1 /CD274 Protein Fc Chimera (Catalog # PD1-H5258) with a linear range of 20 - 200 ng/ml, when detected by HRP labeled Goat anti Human IgG Fc PAb.

This protein is fused with 6×his tag at the C-terminus, and has a calculated MW of 14.3 kDa. The predicted N-terminus is Ala 37. DTT-reduced Protein migrates as 25 kDa in SDS-PAGE due to glycosylation.

-20°C

Gel Pack

1 year

N/A

Lyophilized

Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Centrifuge the vial prior to opening.

For Research Use Only! Not to be used in humans

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Recombinants or rec. proteins

recombinants