Human CellExp Carbonic Anhydrase 9/CA9, human recombinant
Catalog number
7478-10
Name
Human CellExp Carbonic Anhydrase 9/CA9, human recombinant
Size
10 μg
Price
390.00 EUR
Supplier
Details
Synonyms
CAIX, CA9, CA-IX, G250, MN, P54/58N, pMW1
Alternates names
CAIX, CA9, CA-IX, G250, MN, P54/58N, pMW1
Taglines
A zinc metalloenzyme serving as a novel specific biomarker for a cervical neoplasia.
Biomolecule/Target
Carbonic Anhydrase 9/CA9
NCBI Gene ID #.
768
NCBI Gene Symbol
CA9
Gene Source
Human
Accession #
Q16790
Recombinant
Yes
Source
HEK293 cells
Purity
N/A
Activity (Specifications/test method)
N/A
Biological activity
Measured by its esterase activity. The specific activity is measured with 1 mM 4-Nitrophenyl acetate and 2.5 μg enzyme at 400 nm in 100 μL of 12.5 mM Tris, 75 mM NaCl, pH 7.5. The specific activity is > 15 pmoles / min / μg.
Results
Measured by its esterase activity. The specific activity is measured with 1 mM 4-Nitrophenyl acetate and 2.5 μg enzyme at 400 nm in 100 μL of 12.5 mM Tris, 75 mM NaCl, pH 7.5. The specific activity is > 15 pmoles / min / μg.
Molecular Weight
This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 41.2 kDa. The predicted N-terminus is Gln 38. DTT-reduced Protein migrates as 53-54 kDa due to glycosylation.
Storage Temp.
-20°C
Shipping
Gel Pack
Shelf Life
1 year
Concentration
N/A
Appearance
Lyophilized
Physical form description
Lyophilized from 0.22 μm filtered solution in 20 mM MES and 100 mM NaCl, pH 6.5. Normally Mannitol or Trehalose is added as protectants before lyophilization.
Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Handling
Centrifuge the vial prior to opening.
Usage
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants
