Human CellExp Carbonic Anhydrase 10/CA10, human recombinant
Catalog number
7485-10
Name
Human CellExp Carbonic Anhydrase 10/CA10, human recombinant
Size
10 μg
Price
350.00 EUR
Supplier
Details
Synonyms
CA10, Carbonic Anhydrase X, CARPX, hucep-15
Alternates names
CA10, Carbonic Anhydrase X, CARPX, hucep-15
Taglines
Catalyzes the reversible hydration of carbon dioxide in various biological processes.
Biomolecule/Target
Carbonic Anhydrase 10/CA10
NCBI Gene ID #.
56934
NCBI Gene Symbol
CA10
Gene Source
Human
Accession #
Q9NS85
Recombinant
Yes
Source
HEK293 cells
Purity
N/A
Activity (Specifications/test method)
N/A
Biological activity
Measured by its esterase activity. The specific activity, as measured with 1 mM 4-Nitrophenyl acetate (4-NPA) as substrate and 5 μg rh CA10 / CARPX enzyme at 100 ng/µl in 100 μL of 12.5 mM Tris, 75 mM NaCl, pH 7.5. The specific activity is >1 pmol/min/µg.
Results
Measured by its esterase activity. The specific activity, as measured with 1 mM 4-Nitrophenyl acetate (4-NPA) as substrate and 5 μg rh CA10 / CARPX enzyme at 100 ng/µl in 100 μL of 12.5 mM Tris, 75 mM NaCl, pH 7.5. The specific activity is >1 pmol/min/µg.
Molecular Weight
This protein is fused with polyhistidine tag at the C-terminus, has a calculated MW of 32.6 kDa. The predicted N-terminus is Gln 22. DTT-reduced Protein migrates as 37 kDa due to glycosylation.
Storage Temp.
-20°C
Shipping
Gel Pack
Shelf Life
1 year
Concentration
N/A
Appearance
Lyophilized
Physical form description
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH 7.5. Normally Mannitol or Trehalose is added as protectants before lyophilization.
Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Handling
Centrifuge the vial prior to opening.
Usage
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants
