Human CellExp ICAM1 /CD54, human recombinant
Catalog number
7486-20
Name
Human CellExp ICAM1 /CD54, human recombinant
Size
20 μg
Price
350.00 EUR
Supplier
Details
Synonyms
ICAM1, ICAM-1, BB2, BB-2, CD54, CD-54, P3.58
Alternates names
ICAM1, ICAM-1, BB2, BB-2, CD54, CD-54, P3.58
Taglines
A ligand for lymphocyte function-associated (LFA) and Mac-1 integrins
Biomolecule/Target
ICAM1 /CD54
NCBI Gene ID #.
3383
NCBI Gene Symbol
ICAM1
Gene Source
Human
Accession #
P05362
Recombinant
Yes
Source
HEK293 cells
Purity
N/A
Activity (Specifications/test method)
N/A
Biological activity
Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When 5×104 cells/well are added to rhICAM/Fc Chimera coated plates (12.5 μg/ml with 100 μl/well), >60% will adhere after PMA 1 hour incubation at 37ºC.
Results
Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When 5×104 cells/well are added to rhICAM/Fc Chimera coated plates (12.5 μg/ml with 100 μl/well), >60% will adhere after PMA 1 hour incubation at 37ºC.
Molecular Weight
This protein is fused with Fc fragment of human IgG1 at the C-terminus and has a calculated MW of 75.7 kDa expressed. The predicted N-terminus is Gln 27. Protein migrates as 100-110 kDa in reduced SDS-PAGE resulting from glycosylation.
Storage Temp.
-20°C
Shipping
Gel Pack
Shelf Life
1 year
Concentration
N/A
Appearance
Lyophilized
Physical form description
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM glycine, pH 7.0. Normally Mannitol or Trehalose is added as protectants before lyophilization.
Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Handling
Centrifuge the vial prior to opening.
Usage
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants
