Human CellExp PDGFRb/CD140b, human recombinant

Catalog number

7493-10

Size

10 μg

Price

330.00 EUR

Supplier

Biovision

Order

Details

Synonyms

PDGFRB, PDGFR-B, CD140B, CD-140B, JTK12, JTK-12, PDGF-R-beta, PDGFR, PDGFR1, PDGFR-1, PDGFR-beta, PDGFR-β, PDGF-R-β

Alternates names

PDGFRB, PDGFR-B, CD140B, CD-140B, JTK12, JTK-12, PDGF-R-beta, PDGFR, PDGFR1, PDGFR-1, PDGFR-beta, PDGFR-β, PDGF-R-β

Taglines

Binds specifically to PDGFB and has a tyrosine-protein kinase activity

Biomolecule/Target

PDGFRb/CD140b

NCBI Gene ID #.

5159

NCBI Gene Symbol

PDGFRB

Gene Source

Human

Accession #

P09619

Recombinant

Yes

Source

HEK293 cells

Purity

N/A

Activity (Specifications/test method)

N/A

Biological activity

Measured by its ability to inhibit the biological activity of PDGFBB using NR6R-3T3 mouse fibroblast cells. The ED50 for this effect is typically 3-8 μg/ml in the presence of 4 ng/ml rhPDGFBB.

Results

Measured by its ability to inhibit the biological activity of PDGFBB using NR6R-3T3 mouse fibroblast cells. The ED50 for this effect is typically 3-8 μg/ml in the presence of 4 ng/ml rhPDGFBB.

Molecular Weight

This protein contains C-terminal polyhistidine tag and has a calculated MW of 57.1 kDa. As a result of glycosylation, DTT-reduced protein migrates as 85-95 kDa polypeptide in SDS-PAGE.

Storage Temp.

-20°C

Shipping

Gel Pack

Shelf Life

1 year

Concentration

N/A

Appearance

Lyophilized

Physical form description

Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Reconstitution Instructions

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Handling

Centrifuge the vial prior to opening.

Usage

For Research Use Only! Not to be used in humans

Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Additional source

Recombinants or rec. proteins

Group

recombinants