Human CellExp PD-1 /PDCD1, C-Fc Tag, human recombinant

Catalog number
7500-50
Name
Human CellExp PD-1 /PDCD1, C-Fc Tag, human recombinant
Size
50 μg
Price
845.00 EUR
Supplier
Biovision
Order

Details

Synonyms
PDCD1, PD1, CD279, SLEB2, hPD-1, hPD-l
Alternates names
PDCD1, PD1, CD279, SLEB2, hPD-1, hPD-l
Taglines
Inhibits the T-cell proliferation and production of IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway.
Biomolecule/Target
PD-1/PDCD1
NCBI Gene ID #.
5133
NCBI Gene Symbol
PDCD1
Gene Source
Human
Accession #
Q15116
Recombinant
Yes
Source
HEK293 cells
Purity
N/A
Activity (Specifications/test method)
N/A
Biological activity
Measured by its binding ability in a functional ELISA. Immobilized human PD1, c-Fc Protein at 2 μg/ml can bind PD-L1, c-Fc Tag (Catalog # 7429) with a linear range of 0.05–0.5 μg/ml
Results
Measured by its binding ability in a functional ELISA. Immobilized human PD1, c-Fc Protein at 2 μg/ml can bind PD-L1, c-Fc Tag (Catalog # 7429) with a linear range of 0.05–0.5 μg/ml
Molecular Weight
This protein fused with Fc fragment of human IgG1 at the C-terminus, has a calculated MW of 42.6 kDa. The predicted N-terminus is Pro 21. DTT-reduced protein migrates as 50-66 kDa due to glycosylation.
Storage Temp.
-20°C
Shipping
Gel Pack
Shelf Life
1 year
Concentration
N/A
Appearance
Lyophilized
Physical form description
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM glycine, pH 7.5. Normally Mannitol or Trehalose is added as protectants before lyophilization.
Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Handling
Centrifuge the vial prior to opening.
Usage
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants