Human CellExp Osteoactivin / GPNMB, human recombinant
Catalog number
7513-10
Name
Human CellExp Osteoactivin / GPNMB, human recombinant
Size
10 μg
Price
330.00 EUR
Supplier
Details
Synonyms
GPNMB, HGFIN, NMB, Osteoactivin
Alternates names
GPNMB, HGFIN, NMB, Osteoactivin
Taglines
≥95% pure, active, human cell expressed, human recombinant Osteoactivin / GPNMB - acts as a downstream mediator of BMP-2.
Biomolecule/Target
Osteoactivin / GPNMB
NCBI Gene ID #.
10457
NCBI Gene Symbol
GPNMB
Gene Source
Human
Accession #
Q96F58
Recombinant
Yes
Source
HEK293 cells
Purity
N/A
Activity (Specifications/test method)
N/A
Biological activity
Measured by the ability of the immobilized protein to induce pro-MMP-9 secretion by NHLF human normal lung fibroblasts. The ED50 for this effect is typically 4 - 16 µg/ml.
Results
ED50 for this effect is typically 4 - 16 µg/ml
Molecular Weight
This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 52.9 kDa. The predicted N-terminus is Ala 22. DTT-reduced Protein migrates as 85-100 kDa due to glycosylation.
Storage Temp.
-20°C
Shipping
Gel Pack
Shelf Life
1 year
Concentration
N/A
Appearance
Lyophilized
Physical form description
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.
Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in PBS, pH 7.4. Do not vortex.
Handling
Centrifuge the vial prior to opening.
Usage
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants
