Human CellExpCEACAM6/CD66c, human recombinant
Catalog number
7837-10
Name
Human CellExpCEACAM6/CD66c, human recombinant
Size
10 μg
Price
330.00 EUR
Supplier
Details
Synonyms
CEACAM6, CD66c, CEAL, NCA
Alternates names
CEACAM6, CD66c, CEAL, NCA
Taglines
Affects the sensitivity of tumor cells to adenovirus infection. Widely used as a tumor marker in serum immunoassay determinations of carcinomas.
Biomolecule/Target
CEACAM6/CD66c
NCBI Gene ID #.
4680
NCBI Gene Symbol
CEACAM6
Gene Source
Human
Accession #
P40199
Recombinant
Yes
Source
HEK 293 cells
Purity
N/A
Endotoxin Level
< 1.0 EU per μg of the rhCEACAM6 by the LAL method.
Activity (Specifications/test method)
Measured by the ability of the immobilized protein to support the adhesion of calcium ionophore treated human neutrophils. When 2 x 105 cells/well are added to CEACAM6 coated plates (10 μg/mL, 100 μL/well), 45-70% of the cells will adhere after 20 minutes at 37ºC.
Biological activity
Measured by the ability of the immobilized protein to support the adhesion of calcium ionophore treated human neutrophils. When 2 x 105 cells/well are added to CEACAM6 coated plates (10 μg/mL, 100 μL/well), 45-70% of the cells will adhere after 20 minutes at 37ºC.
Results
Measured by the ability of the immobilized protein to support the adhesion of calcium ionophore treated human neutrophils. When 2 x 105 cells/well are added to CEACAM6 coated plates (10 μg/mL, 100 μL/well), 45-70% of the cells will adhere after 20 minutes at 37ºC.
Molecular Weight
This protein rhCEACAM6 is fused with 6×his tag at C-terminus, has a calculated MW of 32 kDa expressed. The predicted N-terminus is Lys35. Protein migrates as 35-65 kDa in reduced SDS-PAGE resulting from glycosylation.
Storage Temp.
-20°C
Shipping
gel pack
Shelf Life
1 year
Concentration
N/A
Appearance
Lyophilized
Physical form description
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.
Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in PBS, pH 7.4. Do not vortex.
Handling
Centrifuge the vial prior to opening.
Usage
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants
