FITC-DEVD-FMK

FITC-Asp-Glu-Val-Asp-fluoromethyl ketone; FITC-D(OMe)-E(OMe)-VD(OMe)-FMK

Liquid

A 0.6 mM solution in DMSO

N/A

FITC-D(OMe)-E(OMe)-VD(OMe)-FMK

C₄₃H₄₆FN₅O₁₅S

923.91

≥98% by HPLC

N/A

Protect from air and moisture

A caspase-3 in situ marker

-20°C

gel pack

36 months

N/A

N/A

Biochemicals

This Biovision Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Biovision FITC is highly sensitive to pH extremes.

Anti-FITC Antibody