Human CellExp PD-L1 /CD274 /B7-H1, human recombinant

Catalog number

7429-250

Size

250 μg

Price

1995.00 EUR

Supplier

Biovision

Order

Details

Synonyms

PD-L1, CD274, B7-H1, PDCD1L1, PDCD1LG1

Alternates names

PD-L1, CD274, B7-H1, PDCD1L1, PDCD1LG1

Taglines

Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner.

Biomolecule/Target

PD-L1 /CD274 /B7-H1

NCBI Gene ID #.

29126

NCBI Gene Symbol

CD274

Gene Source

Human

Accession #

Q9NZQ7

Recombinant

Yes

Source

HEK293 cells

Purity

?90%

Endotoxin Level

N/A

Activity (Specifications/test method)

Measured by its binding ability in a functional ELISA

Biological activity

Immobilized Human PD-L1, His Tag at 5 ?g/mL (100 ?L/well) can bind Human PD-1, His Tag with a linear range of 0.15-1.25 ?g/mL.

Results

N/A

Molecular Weight

This protein is fused with polyhistidine tag at the C-terminus, has a calculated MW of 26.4 kDa. The predicted N-terminus is Phe 19. DTT-reduced Protein migrates as 35-40 kDa due to glycosylation.

Storage Temp.

-20°C

Shipping

Gel Pack

Shelf Life

1 year

Concentration

N/A

Appearance

Lyophilized

Physical form description

Lyophilized from 0.22 ?m filtered solution in PBS pH 7.4

Reconstitution Instructions

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Handling

Centrifuge the vial prior to opening.

Usage

For Research Use Only! Not to be used in humans

Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Additional source

Recombinants or rec. proteins

Group

recombinants