anti-Streptavidin Tag II Antibody

Streptavidin Tag II

Streptavidin Tag II, anti-Streptavidin Tag II, Streptavidin Tag II antibody,Streptavidin Tag II antibody

Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. The original Strep-tag (AWRHPQFGG) was a nine amino acid peptide with high specificity and affinity towards streptavidin which allows the simple purification of protein by use of affinity columns, but required addition to only the C-terminus of recombinant proteins. To also allow a Strep-tag to be placed at the N-terminus of recombinant proteins, it was re-engineered and re-named Strep-tag II.

Rabbit

Polyclonal

Rabbit IgG

All species

A synthetic Streptavidin Tag II peptide NWSHPQFEK conjμgated to KLH.

0.5mg/ml

Liquid

100 µg (0.5 mg/ml) affinity purified rabbit anti-Streptavidin Tag II polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30 % glycerol, 0.5 % BSA, 5 mM EDTA and 0.01 % thimerosal.

Affinity purified

Western blot

The antibody can be used for Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually.

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

anticorps