Target of this antibody:
Alternative names of antibody target:
SERPINH1 , AsTP3 , CBP1 , CBP2 , HSP47 , PIG14 , RA-A47 , PPROM , colligen , colligin-2 , SERPINH2
Heat shock proteins (HSPs) are ubiquitously expressed in all organisms. HSP47 is a molecule expressed constitutively in cells that synthesizes collagen. HSP47 is a procollagen-specific molecular chaperon that resides in the endoplasmic reticulum of procollagen-producing cells and is essential for secretion of procollagen from cells. Significantly, HSP47 plays a vital role in folding and assembling collagen. HSP47 is also known as colligin 1, serpinh1, collagen-binding protein 1 (CBP1) and gp46. The human HSP 47 gene encodes a 47 kDa protein.
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat, chicken
Synthetic peptide surrounding amino acid 407 of human HSP47
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.2 mg/ml) Protein A purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blot, Immunoprecipitation, Immunohistochemistry
Western blotting (0.5-4 µg/ml), immunoprecipitation (10-20 µg/ml) and Immunohistochemistry (10-20 µg/ml) in frozen or paraffin embedded tissues. However, the optimal concentrations should be determined individually. The antibody recognizes 47 kDa Hsp47 of human, mouse, rat, and chick origins. Mouse small intestine tissue lysate (Cat.# 2601-100) can be used as a positive control.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.