Target of this antibody:
Alternative names of antibody target:
GSK-3 is a serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 has been implicated in the regulation of cell fate and in the Wnt signaling pathway. GSK-3 plays an important role in the PI3 kinase and Akt mediated cell survival pathways, and its activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β.
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat
Synthetic phospho-peptide derived from human GSK-3a
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) affinity-purified rabbit polyclonal phospho-GSK-3a(Ser21/9) polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% sodium azide.
This antibody has been succesfully tested for use in following methods:
Western blot, Immunohistochemistry
Western blot analysis (1-2 µg/ml), and Immunohistochemistry (20-30 µg/ml). However, the optimal conditions should be determined individually. The phospho-GSK-3α/β (Ser21/9) antibody detects GSK-3 only when phosphorylated at Ser21 of GSK-3α or Ser9 of GSK-3β.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.