• CONTENTS: Supplied as a 50% slurry in 20% ethanol/H₂O;>2.5 mg heparin per ml Sepharose beads. • FEATURES: Heparin-beads have been widely used in affinity purification of various heparin-binding proteins or ligand, such as antithrombin III, lipoprotein, DNA binding proteins (transcription factors, virus coat proteins etc.) and other ligands. BioVison’s Heparin-Sepharose is designed for the purpose of purification of these kinds of proteins and ligands. It can also be used as a high capacity cation exchange medium. Specific proteins can be separated by using different concentrations of salt or a salt gradient. This Heparin-Sepharose formulation exhibits excellent binding capacity, high flow rate, no significant loss of the heparin ligand and a pH stability range of 2-10. • APPLICATIONS: Purification of heparin-binding proteins, enzymes or other ligands.
Heparin-Sepharose is prepared by covalently coupling heparin to epoxy-activated 6% cross-linked sepharose beads. The coupling was optimized to give a high binding capacity and could be greater than 0.4 mg of heparin-binding protein (such as thrombin) per ml of wet gel.
Maximum time for storage